Abstract: In recent years, the decline disease has seriously damaged the healthy development of Morella rubra industry. In order to develop the effective prevention and control measures, the mixed fertilization experiment was carried out on the Myrica rubra with facility cultivation in winter. The bio-organic fertilizer 20 kg·plant^-1+anti-continuous cropping microbiological preparation 0.2 kg·plant^-1 (treatment T) was applied in the dripping line of Myrica rubra. And the conventional fertilization was used as the control group (CK) to determine the related traits of leaves and fruits of Myrica rubra trees in the mature season. The results showed that the mixed fertilization (treatment T) could effectively improve the vegetative growth and fruit quality of weak Myrica rubra. Compared with the control group (CK), the mixed fertilization significantly promoted the growth of shoot length and leaf length of the diseased trees, and increased the chlorophyll content by 62.60%, 10.28% and 14.68%, respectively. At the same time, the mixed fertilization (treatment T) significantly increased the contents of total polysaccharide, vitamin C, total flavonoids and anthocyanins in fruit by 7.99%, 59.38%, 4.46% and 10.81%, respectively, compared with the control group (CK). In addition, the mixed fertilization (treatment T) inhibited the production of total acid in fruit, which was significantly reduced by 18.74% compared with the control group (CK). The application of bio-organic fertilizer combined with the anti-continuous cropping microbial agents could promote the vegetative growth of weak Myrica rubra and improve the taste of fruit.