大豆拉丝蛋白改善鱼糜质构的工艺优化

    Process Optimization of Textured Soybean Protein for Enhancing the Textural Properties of Surimi Gels

    • 摘要: 为有效改善鱼糜质构品质,以大豆拉丝蛋白(TSP)为改良剂,通过单因素试验结合响应面法优化鱼糜质构改善工艺,探究其添加量、蛋白酶预处理及不同加工参数等对鱼糜质构的影响。结果表明:随TSP添加量增加(湿基0~30%),鱼糜硬度、弹性、凝胶强度、持水性等呈先升后降趋势,白度值则持续降低。TSP最佳添加量为10%,此时鱼糜凝胶的质构指标达峰值。此外,TSP经木瓜蛋白酶(100 U·g−1、60℃、30 min)预处理可显著提升鱼糜凝胶强度;添加0.12 U·g−1的TG酶可明显改善其持水性。同时,通过响应面分析确定最优工艺参数为:在木瓜蛋白酶预处理的基础上,TSP添加量10.5%、凝胶化时间35 min、蒸煮时间35 min;此条件下预测鱼糜凝胶强度为4 633.87 g·mm,验证试验实测值4 582.27 g·mm,相对误差<1.2%,模型可靠。

       

      Abstract: In order to effectively improve the textural quality of surimi, by using the Textured Soybean Protein (TSP) as modifier, the texture improvement process of surimi was optimized through the single factor experiment combined with the response surface method, and the effects of TSP addition level, protease pretreatment and different processing parameters on the texture of surimi were investigated. The results showed that with the increase of TSP addition (0 ~ 30% wet basis), the hardness, springiness, gel strength and water holding capacity of surimi initially increased and then decreased, while the whiteness value continuously declined. The optimum addition amount of TSP was determined to be 10%, at which the textural parameters of surimi gel reached their maximum values. Additionally, the pretreatment of TSP with papain (100 U·g−1, 60℃, 30 min) could significantly improve the gel strength of surimi. Furthermore, the supplementation with 0.12 U·g−1 transglutaminase (TGase) could markedly improve its water holding capacity. Through the response surface analysis, the optimal processing parameters were identified as follows: on the basis of papain pretreatment, TSP addition level of 10.5%, gelation time of 35 min, and cooking time of 35 min. Under these conditions, the predicted gel strength of surimi was 4 633.87 g·mm, while the measured value of validation experiments was 4 582.27 g·mm, with the relative error of less than 1.2%, confirming the reliability of the model.

       

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