Abstract: Effects of different fertilizer amount and fertilizer application frequency on tealeaves output, one-hundred-bud weight, budding density and soil physical and chemical properties by applying organic fertilizer of humic acid in tea garden were studied. The results showed that the tealeaves output increased with the increase of the applying dosage of humic acid fertilizer, the increased range was 4.32%-12.62%. In addition, applying humic acid fertilizer could increase budding density and one-hundred-bud weight of tea plant, and improve the soil pH value by 4.50%-14.19%. Based on the above results, it was suggested that, application amount of humic acid fertilizer was 40 kg/667m² (600 kg/hm²) with single application.