Abstract: In an artificial breeding setting, the striped bamboo sharks exhibited abnormal behavior, agitation, and mortality. In order to identify the causative pathogen of the disease and screen for effective therapeutic agents, a dominant bacterial strain designated CV-P1 was isolated from the spleen tissue of affected sharks. Through the morphological observation, physiological and biochemical characterization, 16S rRNA gene sequencing, phylogenetic tree construction, and recurrent infection tests, the strain CV-P1 was comprehensively identified as Vibrio harveyi, with a median lethal dose (LD₅₀) of 1.0×10⁵ CFU·mL⁻¹. The drug susceptibility test indicated that the bacterium was susceptible to 12 antibiotics, including enrofloxacin, neomycin, and thiamphenicol, moderately susceptible to colistin, and resistant to 9 antibiotics, such as florfenicol and tetracycline. Furthermore, the minimum inhibitory concentration (MIC) of each antimicrobial agent against the strain CV-P1 was determined by using the microdilution method. The results indicated that flumequine, sulfafurazole, ceftiofur, ceftazidime, and ofloxacin all had an MIC of 0.25 μg·mL⁻¹; while the MIC values for enrofloxacin, neomycin, thiamphenicol, doxycycline, sulfamonomethoxine, augmentin, and gentamicin were 0.03, 2, 1, 0.125, 2, 2/1, and 0.5 μg·mL⁻¹, respectively. This study represented the first report of V. harveyi isolated from the diseased striped bamboo sharks, and the results could provide scientific reference for the prevention, control and treatment of bacterial diseases in this species.