Abstract: In order to clarify the regulatory mechanisms of Dictyophora indusiata cultivation on the soil phosphorus cycle in paddy fields, the Dictyophora indusiata cultivation plots (JXZS) and the control plots (JXCK) were selected as the experimental subjects. The effects of Dictyophora indusiata cultivation on the soil phosphorus cycle were systematically explored by measuring the soil phosphorus content and the key enzyme activity of phosphorus cycle, combined with the functional genomics analysis. The results showed that: Dictyophora indusiata cultivation increased the soil total phosphorus (TP) content by 54.7% and the available phosphorus (AP) content by 137.5%, concurrently activating the acid phosphatase (ACP) activity by 33.0%. Phytase (PHY) activity exhibited a significant increase, whereas the phosphodiesterase (PDE) activity remained unchanged. (2) The microbial phosphorus metabolic strategy shifted from a "low-phosphorus adaptation" phenotype towards a "high-efficiency utilization" phenotype. The genes such as phosphorus storage (ppk2) and recalcitrant organic P utilization (phnP), alongside the enzyme systems including EC 3.1.4.55 (complex organic P degradation) were enriched in the control plots. In contrast, the E3.1.3.8 gene (easily degradable organic phosphorus mineralization) and its corresponding enzyme system were enriched in the cultivation plots. (3) The soil enzyme activities (PHY and PDE) were the key factors driving the microbial functional variation, which were significantly negatively correlated with the core genes such as ppx-gppA, ppa, while the total phosphorus content exhibited no significant regulatory effect on microbial functionality. (4) The P metabolic network showed the structural characteristics of “loose structure with core node-driven” topology. Within the gene network, only two connector nodes (phnO, E3.1.3.1/phoA/phoB) were identified. E3.1.3.8 and phoN were the core functional genes within the bipartite network, while the majority of microbial genera constituted the peripheral nodes. In conclusion, Dictyophora indusiata cultivation systematically reshaped the phosphorus metabolic functional pattern of soil microorganisms by increasing the soil phosphorus pool capacity and activating the key phosphatases in farmland. This process has led to a shift in microbial strategy from relying on the endogenous phosphorus reserves to prioritizing the utilization of exogenous easily degradable organic phosphorus. The optimization of this metabolic strategy was an important microbiological mechanism for the improvement of soil phosphorus cycling efficiency in Dictyophora indusiata cultivation plots.