一个水稻重组自交系群体的全基因组选择模型分析

    Genome-wide Selection Model Analysis of a Rice Recombinant Inbred Line Population

    • 摘要: 为了更好地在育种中利用一个由蜀恢527和绵恢436杂交构建的重组自交系(RIL)群体,建立了该群体的全基因组选择(GS)模型,并评估出最优模型用于将来筛选强优恢复系,以提高育种效率。在检测了该群体数量农艺性状及基因型后构建了3个GS模型,通过评估GS模型在RIL群体的各类性状上的表现,探究了3个预测模型在该群体中应用的准确性和预测能力。结果表明:水稻RIL群体的抽穗期、有效分蘖数、株高、粒长、粒宽、穗长、每穗实粒数、单株产量等8个性状均为多基因控制的数量性状,可用于开展GS分析工作。利用高通量测序获得群体的基因型,经筛选过滤后,获得了181份样本的454 129个SNPs并构建了3个预测模型。从模型的准确度和预测能力两个方面评估,GBLUP模型总体表现优秀可用于后续使用。

       

      Abstract: In order to make better use of a recombination inbred line (RIL) population derived from the cross of Shuhui 527 and Mianhui 436 in breeding, the genome-wide selection (GS) model for this population was constructed, and the optimal model was evaluated for the future screening of superior restorer lines to improve the breeding efficiency. Three GS models were constructed after detecting the agronomic traits and genotypes of the population quantity. Then, by evaluating the performance of GS model on various traits of the RIL population, the accuracy and predictive ability of the three prediction models applied in this population were explored. The results showed that: the eight traits such as the ear sprouting period, effective tiller number, plant height, grain length, grain width, panicle length, grain number per panicle and yield per plant of rice RIL population were all the quantitative traits controlled by polygenes genetically, making them suitable for GS analysis. The genotypes of the population were obtained by high-throughput sequencing. After screening and filtering, 454 129 SNPs from 181 accessions were obtained and three predictive models were constructed. The comprehensive evaluation based on the model accuracy and predictive capacity revealed that the GBLUP model exhibited superior overall performance, demonstrating its potential for practical implementation in the subsequent use.

       

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