Tissue culture and rapid propagation technique for Actinidia chinensis

Effects of different treatments on explant sterilization, different hormone formulation on initial culture of explant and rooting of tissue culture seedling, were studied, using Actinidia chinensis stem with axillary bud as explant. The results showed that the best sterilizaiton treatment was 75% alcohol for 30 s firstly, and then 0.1% Mercury(II) chloride for 8 min, and the survival rate of explant reached 88%. The optimum initial culture medium was MS+6-BA 1.5 mg/L+NAA 0.2 mg/L, the budding rate of which was 92%. The optimum rooting culture medium was 1/2 MS+IBA 0.15 mg/L+NAA 0.3 mg/L, with 85.6% of the rooting rate.