Optimization of Protein Extraction Technology for Grifola Frondosa

An orthogonal experimental design was used to optimize the protein extraction technology for Grifola frondosa. The results showed that the influence of extraction factors on the index was decreased in order C > D > B > A. The optimal extraction technology included, the ratio of material to liquid was 1∶50, the extracting temperature was 45℃, the pH value was 10, and 5 times of the cells ultrasonic crush, and under these conditions, the extraction rate for protein of Grifola frondosa reached 0.95%.