Determination of Benzoapyrene Residue in Stir-fried Nuts by Solid Phase Extraction and High Performance Liquid Chromatography Fluorescence Spectrometry

To establish a method for determing benzoapyrene residue in stir-fried nuts by HPLC-fluorescence detector, the sample was extracted with n-hexane and acetone (V:V=3:1) mixed solvent, purified by neutral alumina column and determined by HPLC equipped with fluorescence detector. The results showed that the linearity of benzoapyrene was good in the range of 0.50-10.00 ng·mL^-1 with the linear equation y=119649 x+1831.08 and the correlation coefficient 0.99998. The average recovery was 80.4%-87.2% and the relative standard deviation was 3.5%-4.2% when standard samples were added at 3 different concentration levels (1.00, 5.00, 10.00 ng·mL^-1). The results showed that the method was sensitive, simple and accurate, and could meet the requirement for the determination of benzoapyrene residues in stir-fried nuts.