Studies on Cutting Seedling Raising Techniques of Dendrobium officinale

In this paper, some practices, such as material selection for cutting, cutting disinfection, treatment with plant growth regulators and substrate ratio of Dendrobium officinale were compared to probe cutting seedling raising techniques so as to improve its cutting sprouting rate. The results showed that the sprouting rate reached 80.0%-85.5% when young tissues above pseudobulbs were used as cuttings, immersed into the suitable combination of plant growth regulator of TDZ 200 mg·L^-1+NAA100 mg·L^-1+IBA200 mg·L^-1 and placed in sphagnum substrate after 30days. Thus it was helpful in bud differentiation.