Study on the Technique of in Vitro Conservation for Mosaic Anoectochilus Formosanus

The method of slow growth conservation was used to conduct the experiment of in vitro conservation of mosaic anoectochilus formosanus. The results showed that:In the culture conditions of the temperature being 24±3℃, the illumination intensity being 1 500-2 000 lx for the first 3 months and 500-1 000 lx after 3 months, and the illumination time being 10 h·d^-1, the survival rate of the single bud of mosaic anoectochilus formosanus reached 85.0% after 12 months conservation in the modified MS+sucrose 20 g·L^-1+ mannitol 20 g·L^-1 medium. And it could restore normal growth after the conservation, which provided a new method and idea for the germplasm conservation of anoectochilus formosanus.