Genomic Features of Salipiger sp. D13 and its Degradation Ability on Phthalates

In order to clarify the degradation characteristics of the strain of Salipiger sp. D13 on phthalates, the genomic sequence of Salipiger sp. D13 and the degradation ability of this strain on phthalates were analyzed. Meanwhile, according to the key enzyme gene and intermediate metabolites, the complete pathway of Salipiger sp. D13 to metabolize DBP was speculated. The results showed that the strain of Salipiger sp. D13 contained the key enzyme genes for the degradation of phthalates, which were distributed in the metabolic pathways such as the degradation of polycyclic aromatic hydrocarbons and the metabolism of benzoic acid, and the 5-day degradation rates of the six priority controlled PAEs by the strain of Salipiger sp. D13 were all above 90%. The complete pathway of Salipiger sp. D13 to degrade dibutyl phthalate was that: dibutyl phthalate was hydrolyzed to phthalic acid (PA) by esterase.3, and PA produced protocatechuic acid (PCA) by oxygenase, dehydrogenase and decarboxylase, and then PCA was hydrolyzed to the substances such as β-carboxy-muconate by ring opening reaction with protocatechuate 3,4-dioxygenase, which were then delivered to the tricarboxylic acid cycle. The experimental results could provide a theoretical basis for the application of Salipiger sp. D13 as a candidate strain for the environmental bioremediation of phthalate ester pollutants.