Construction of the Overexpression Plasmid of MpigE Gene in Monascus

By taking the M-CL of Monascus in Fujian as the original strain, the PtrpC promoter and TrpC terminator were cloned by using the PSKH plasmid as the template. The PMT fusion fragments were obtained by fusing PtrpC promoter, MpigE target gene and TrpC terminator with the overlap-extension PCR technology. In order to verify the correctness of the fusion fragment PMT, the agarose gel electrophoresis analysis was carried out, and a single clear band appeared at about 2,100 bp. The fragment of PMT was digested by EcoR V and Spe I, and the EcoR V and Spe I sites of the PSKH plasmid with hydthromycin resistance were inserted to construct an overexpression vector HPMT of Monascus, with a size of 7 155 bp. By constructing the overexpression vector, the M-CL of Monascus was modified, thus to obtain the strain with high yield of pigment and low yield of citrinin.