Optimization of the Extraction Process of Total Flavonoids from Hypsizygus marmoreus with Oscillation Technique by Using the Response Surface Method

In order to optimize the technological conditions of total flavonoids extracted from Hypsizygus marmoreus by oscillation method and improve the yield of total flavonoids, the single factor experiment was designed with ethanol as the solvent to explore the effects of solid-liquid ratio, extraction temperature, extraction time and ethanol volume fraction on the yield of total flavonoids from Hypsizygus marmoreus, and the response surface optimization experiment was carried out on this basis. The results showed that under the conditions of ethanol volume fraction of 50%, solid-liquid ratio of 1∶30, extraction temperature of 59℃ and extraction time of 2 h, the yield of total flavonoids from Hypsizygus marmoreus was 3.22 mg·g^-1.