Process Optimization of the Extraction of Nuciferine by Cellulase Enzymatic Hydrolysis

In order to optimize the process conditions of extracting the nuciferine in lotus leaves by cellulase enzymatic hydrolysis, the effects of the adding amount of cellulase, enzymatic temperature, enzymatic time and the ratio of liquor to material on the extraction rate of nuciferine in lotus leaves were investigated by using the method of combining the single factor test and orthogonal test. The best technological conditions for the extraction of nuciferine in lotus leaves by cellulase were as follows: the adding amount of cellulase was 2.3 mg·g^-1, the enzymatic temperature was 50℃, the enzymatic time was 55 min, and the ratio of liquor to material (mL/g) was 30∶1. Under these conditions, the extraction rate of nuciferine in lotus leaves could be as high as 0.67%. The research results could provide positive reference value for the subsequent development and utilization of nuciferine in lotus leaves.