Identification of the Chorismic Acid Synthetase Gene BbaroC in Brevibacillus brevis and Analysis of the Transcription Factor Regulation

The chorismic acid synthetase AroC is the key enzyme for the synthesis of aromatic compounds in the shikimic acid pathway. Identifying the characteristics of aroC gene in Brevibacillus brevis and its transcriptional regulators will lay a foundation for the study of the mechanism of action of Brevibacillus brevis. The aroC gene was cloned from Brevibacillus brevis FJAT-0809-GLX, and then the bioinformatics analysis and the prediction of transcription factor were carried out. The sequence length of aroC gene in Brevibacillus brevis FJAT-0809-GLX was 1164 bp, and the GenBank accession number was OR475562. The aroC gene sequence of Brevibacillus brevis FJAT-0809-GLX had the highest homology with that of Brevibacillus brevis NBRC 100599, which was 98.88%. The amino acid sequence of AroC had the highest homology with Brevibacillus brevis HNCS-1, which was 100%. Its encoded protein was a hydrophilic protein without signal peptide, which was located in the cytoplasm. A total of 23 transcription factors targeting BbaroC were identified from 10 species, with 12 and 3 transcription factors predicted from Escherichia coli (Strain K12 MG1655) and Bacillus subtilis (Strain 168), respectively. BbaroC may play an important role in the production of antibacterial active substances of Brevibacillus brevis, which would provide basis for its wide application in agriculture.