Diversity analysis of endophytic bacteria in Kandelia candel based on 16S rDNA sequence

To study the diversity of culturable endophytic bacteria in the roots, branches and leaves of Kandelia candel in the wetland of Minjiang River estuary, and to provide support for the study of community structure, distribution, stability and functional endophytic bacteria of Kandelia candel. In this study, culturable endophytic bacteria were isolated from different tissues of Kandelia candel, and the endophytic bacterial diversity was analyzed based on 16S rDNA gene sequence alignment and phylogeny. The results showed that 36 strains of culturable endophytic bacteria were isolated from three tissues of Kandelia candel, which belongs to 11 genera and 22 species（2 strains among which are not officially named）. Bacillus and Vibrio were the dominant strains, accounting for 21.2% and 19.4% of the total strains, respectively. There were differences in the distribution and diversity of endophytic bacteria in different tissues, among which the number of endophytic bacteria was the highest in roots（27 strains, 75% of the total number of strains）, followed by branches（6 strains, 16.7% of the total number of strains）, and the least in leaves（3 strains, 8.3% of the total number of strains）. The endophytic bacteria of Kandelia candel have rich species diversity in the wetland of Minjiang River estuary, and the diversity of endophytic bacteria in the root is the most abundant.