Effect of lactobacillin(PlnJ(G5)）on intestinal inflammation induced by LPS

To explore the effect of Lactobacillin PlnJ(G5）on LPS-induced lower intestinal epithelial cells IPEC-J2 and intestinal inflammation in mice. Enteric epithelial cells IPEC-J2 and model animal mice were selected as study carriers. The inflammation model was established using LPS and treated with PlnJ(G5). cck-8 was used to detect cell viability and real-time fluorescence quantitative PCR was used to detect the expression changes of inflammation-related cytokines. The results show that: LPS 5.0 μg·mL⁻¹ had little effect on the viability of IPEC-J2 cells, and the mRNA expressions of IL-1, IL-6, TNF-α, IFN-α and IFN-β in IPEC-J2 cells were significantly higher than those in the control group, indicating that LPS successfully induced inflammation modeling in IPEC-J2 cells. The results of CCK-8 detection showed that the safe dosage of PlnJ(G5）for IPEC-J2 cells was 20.0 μg·mL⁻¹. The results of qPCR showed that the mRNA expression of IL-1, IL-6, TNF-α, IFN-α and IFN-β in IPEC-J2 cells was best inhibited by adding 20.0 μg·mL⁻¹ PlnJ(G5）without heat treatment after being treated with LPS 5.0 μg·mL⁻¹ for 4h. The experimental results showed that PlnJ(G5), which was not heat treated, had the best anti-inflammatory effect on mice induced by LPS.