Effect Evaluation on Different Immune Procedures of Avian Reovirus Vaccine

In order to study the antibody growth and elimination of avian reovirus (ARV) commercial vaccine under different immune procedures and its effect on the production performance of chickens, the immune procedure 1 (live vaccine + 2 times inactivated vaccine) and immune procedure 2 (live vaccine + 3 times inactivated vaccine) were set up. Then, the positive rate and titer of serum antibody in chickens were detected by using ELISA method, and the mortality rate, egg production rate and weight gain of chickens were evaluated to screen the better immune procedure. The results showed that the antibody growth-decline rule under the immune procedure 1 and immune procedure 2 were basically the same. The antibody reached the maximum at 21 weeks of age, and then maintaining at a relatively high level. The antibody titer under the immune procedure 2 reached 1.91×10⁴ at 21 weeks of age, while the maximum value under the immune procedure 1 was only 1.42×10⁴, and the antibody level was significantly lower than that under the immune procedure 2 at the later stage. During the brooding and rearing period, the mortality rate of parental generation chickens under the immune procedure 2 was 4.38%, which was lower than that under the immune procedure 1 (6.34%) , and the peak egg production rate was increased by 1.43% compared with that under the immune procedure 1. The further statistical analysis of maternal antibodies in offspring commercial broiler chickens showed that the mean value of maternal antibody in chickens immunized with program 2 was 8.53×10³, which was significantly higher than that immunized with program 1 (6.76×10³). Meanwhile, the mortality rate of offspring commercial broilers under the immune procedure 2 was 0.43% (0.49% / 0.92%) lower than that under the immune procedure 1, and the weight gain of broilers at 1 week old was about 12 g (164 g/152 g). The above data showed that the immune procedure 2 was superior to the immune procedure 1 in both the level of antibodies induced and the production performance of chickens. These results provided reference for the formulation of immunization schedule of avian reovirus vaccine for poultry farms in China.