Establishment of Tissue Culture and Rapid Propagation System for Fresh Cassava Huanan No. 9

In order to establish the tissue culture and rapid propagation technical system of cassava Huanan No.9, the apical buds and stem segments with axillary buds of fresh cassava Huanan No.9 were used as the explants to improve the MS basic medium, and the self-created basic medium ZCM was designed. Then, the effects of adding different concentrations of 6-BA and IBA to the medium on the proliferation and rooting were studied, and the effects of refining seedling methods on the survival rate of transplanting were also discussed. The results showed that: the explants cultured on MS and ZCM medium could induce the germination and growth, and the induction rates of aseptic seedlings from stem segments and apical buds were 66.7% and 46.7%, respectively. It was better to use the stem segments with axillary buds as the explants. The suitable medium for proliferation culture was MS and ZCM, and the proliferation coefficients were 3.5 and 3.4, respectively. The suitable rooting culture medium was MS and ZCM, with the rooting rate reaching 100%, and the number of roots was large and long. Therefore, the survival rate of transplanting could be effectively improved by using the refining seedling method of first out of the bottle and then liquid culture in the tissue culture of root seedlings.